Thursday, March 5, 2015

Anxiety: An Overview



 Anxiety: An Overview



Appropriate levels of anxiety, commonly referred to as fear or worry, is a normal mental process that has remained with humans through evolution because its presence has protective and life surviving traits. Fear and anxiety create a behavioral response that prevents an individual from entering situations that are likely to cause injury, pain and possibly death. Anxiety is peaked when there is a feeling on impending danger and perceptions stimulant the brain to be on high alert and prepare for the “fight or flight” response.
The uncomfortable feeling of anxiety prevents an individual from entering environments that may be dangerous to our well-being, such as steep rocky cliffs or “bad” neighborhoods. However, some have neurological settings that trigger anxiety at low levels of danger. Less dangerous environments, or simply even thoughts, in these individuals can prematurely generate anxiety that interferes with ones life. For example, if someone has an excessive fear of heights, they may not seek employment or attend a school that requires them to be on higher floors. The evolutionary fear of heights was important so early humans would not pursue prey or food in elevated levels where there was a significant risk of falling, injury or death. Anxiety has an important role in the maintenance for our well-being, but excessive anxiety can significantly impair an individual’s life.
It is often difficult to determine the precise difference between appropriate levels of anxiety and those that are pathological. Essentially, a level of fear or anxiety are considered abnormal if persistent, intense and without real danger present. When the intensity and duration of the presence of fear and anxiety is disproportional, or is not even associated with a genuine risk, this results in a debilitating state of prolonged anxiety. Individuals experiencing pathological anxiety display signs of excessive worrying, restlessness, fatigue, irritability, muscle tension, along with concentration and sleep disturbances. Physical symptoms include tachycardia, high blood pressure, sweating, dry mouth and dilated pupils.
Anxiety disorders are a group of mental health conditions that include: generalized anxiety disorder (GAD), social anxiety, post-traumatic stress disorder (PTSD) and panic disorder.[1] A diagnosis of anxiety disorder is warranted when the extent of the anxiety interferes with thought processes and behavior that prevents an individual from performing daily activities and maintaining relationships. The two most predictive indicators to determine if the level of anxiety is greater than normal is; 1) determining the percent of the day in worry, and 2) the number of areas one worries about.[2] Any one of the anxiety conditions can lead to reduced life experiences and impair ones ability to function in society.
Anxiety is generated and propagated in specific neural circuits in the brain, which subsequently activates several organ systems in the body to prepare the body for something harmful to happen. When anticipating physical danger, the brain prepares the body to fight to survive, or if the stimulus too great (a bear), to prepare to run to safety (flight). In preparation for these physical events, neuroendocrine signals stimulate the heart to increase the rate and amount of oxygenated blood pumped to muscles, increases the release of epinephrine (adrenaline) to the sympathetic nervous system and steroids from adrenal glands, and increases perspiration to cool the body.[3]
This manuscript will use the term anxiety to represent any level of excessive fear, stress or anxiety that causes mental anguish and limits ones ability to enjoy and freely participate in their lives.
Epidemiology
Anxiety disorders are most common of mental health conditions. A systematic meta-analysis, which included 87 studies from 44 countries, reported that the average incidence of anxiety disorders was 15%.[4] In the U.S., a 2010 prospective study found the lifetime prevalence of GAD was 14.2%. [5] An Australian study in 2014, found that the lifetime prevalence of anxiety disorders occurred in 20% of the population with an average age of symptom onset at 19 years. The most common risk factors for developing an anxiety disorder are: female, single, unemployed, having a physical health condition and a family history of mental health disorders. [6]
This reported incidence of anxiety disorders does not include individuals with mild to moderately elevated anxiety levels, referred to as trait anxiety. This group does not meet the criteria for an anxiety disorder, but their lives are detrimentally affected by excessive anxiety.  The incidence of trait anxiety is believed to be significantly growing in today’s global, high-tech, fast paced societies.


The understanding of the neurochemical etiology of anxiety has evolved tremendously over the past two decades. Serotonin, g-aminobutyric acid (GABA), glutamate and norepinephrine neurotransmitter activity and specific receptors in key areas of the brain have received the greatest amount of research. Clinical, biochemical, radiological and molecular imaging studies have consistently documented the important role neurotransmitters play in the modulation of mood and anxiety.

Risk Factors
Risk factors for developing excessive anxiety are a combination of environmental, social, neurological, medical and genetic factors. Poor living conditions, abuse, bereavement, divorce/separation, depression, comorbid medical or neurological disorders and family history of anxiety are the most commonly associated risk factors.[7] [8] [9] [10] Recent research indicates that neurotransmitter activity changes are key components in generating the emotional symptoms and physical consequences of anxiety.
Those with neurological conditions are at increased risk for developing an anxiety disorder. The impaired function responsible for a given neurological disorder may influence the modulation of anxiety related neural circuits. The most common neurological disorders associated with a high rate of co-morbid anxiety are epilepsy and multiple sclerosis.[11]

Associated Disorders
There is a significant literature confirming that anxiety, and even anxiety trait, impair cognitive functions. These functions include reduced goal-directed sustained attention,[12] prefrontal brain attention control [13] and ability to inhibit or block out distractions. [14]
Anxiety and high levels of perceived stress have been shown to disrupt the body’s immune function [15] [16] by reducing the actual number of white blood cells (WBC) and the cytokines that provide the signals for specific infections. [17] These factors can lead to suppress the immune system and increase the risk of infections.
Anxiety increases the risk of developing several physical and mental conditions and diseases. GAD was found to be highly associated with cardiovascular deaths in recent studies.[18] [19] [20] Over a 20-year monitoring study, it was determined that negative health outcomes were proportionately related to the degree of persistent anxiety.[21] In addition, anxiety is comorbid and often complicates survival in many conditions such as cardiovascular disease, depression, Parkinson’s, multiple sclerosis, epilepsy and schizophrenia. [22]
   [23] [24] [25]
References


1.             Marnane, C. and D. Silove, DSM-5 allows separation anxiety disorder to grow up. Aust N Z J Psychiatry, 2013. 47(1): p. 12-5.
2.             Niles, A.N., et al., Dimensional indicators of generalized anxiety disorder severity for DSM-V. J Anxiety Disord, 2012. 26(2): p. 279-86.
3.             Veen, G., et al., Basal cortisol levels in relation to dimensions and DSM-IV categories of depression and anxiety. Psychiatry Res, 2011. 185(1-2): p. 121-8.
4.             Baxter, A.J., et al., Global prevalence of anxiety disorders: a systematic review and meta-regression. Psychol Med, 2013. 43(5): p. 897-910.
5.             Moffitt, T.E., et al., How common are common mental disorders? Evidence that lifetime prevalence rates are doubled by prospective versus retrospective ascertainment. Psychol Med, 2010. 40(6): p. 899-909.
6.             McEvoy, P.M., R. Grove, and T. Slade, Epidemiology of anxiety disorders in the Australian general population: findings of the 2007 Australian National Survey of Mental Health and Wellbeing. Aust N Z J Psychiatry, 2011. 45(11): p. 957-67.
7.             Maniglio, R., Child sexual abuse in the etiology of anxiety disorders: a systematic review of reviews. Trauma Violence Abuse, 2013. 14(2): p. 96-112.
8.             Mathew, A.R., et al., Co-morbidity between major depressive disorder and anxiety disorders: shared etiology or direct causation? Psychol Med, 2011. 41(10): p. 2023-34.
9.             Pajak, A., et al., Depression, anxiety, and risk factor control in patients after hospitalization for coronary heart disease: the EUROASPIRE III Study. Eur J Prev Cardiol, 2013. 20(2): p. 331-40.
10.          Espejo, E.P., C. Hammen, and P.A. Brennan, Elevated appraisals of the negative impact of naturally occurring life events: a risk factor for depressive and anxiety disorders. J Abnorm Child Psychol, 2012. 40(2): p. 303-15.
11.          Torta, R. and R. Keller, Behavioral, psychotic, and anxiety disorders in epilepsy: etiology, clinical features, and therapeutic implications. Epilepsia, 1999. 40 Suppl 10: p. S2-20.
12.          Eysenck, M.W., et al., Anxiety and cognitive performance: attentional control theory. Emotion, 2007. 7(2): p. 336-53.
13.          Bishop, S.J., Trait anxiety and impoverished prefrontal control of attention. Nat Neurosci, 2009. 12(1): p. 92-8.
14.          Beaudreau, S.A. and R. O'Hara, The association of anxiety and depressive symptoms with cognitive performance in community-dwelling older adults. Psychol Aging, 2009. 24(2): p. 507-12.
15.          Leonard, B.E. and C. Song, Stress and the immune system in the etiology of anxiety and depression. Pharmacol Biochem Behav, 1996. 54(1): p. 299-303.
16.          Bloom, B.L., S.J. Asher, and S.W. White, Marital disruption as a stressor: a review and analysis. Psychol Bull, 1978. 85(4): p. 867-94.
17.          Farrar, W.L., Evidence for the common expression of neuroendocrine hormones and cytokines in the immune and central nervous systems. Brain Behav Immun, 1988. 2(4): p. 322-7.
18.          Martens, E.J., et al., Scared to death? Generalized anxiety disorder and cardiovascular events in patients with stable coronary heart disease:The Heart and Soul Study. Arch Gen Psychiatry, 2010. 67(7): p. 750-8.
19.          Roest, A.M., et al., Anxiety and risk of incident coronary heart disease: a meta-analysis. J Am Coll Cardiol, 2010. 56(1): p. 38-46.
20.          Denollet, J., et al., Anxiety predicted premature all-cause and cardiovascular death in a 10-year follow-up of middle-aged women. J Clin Epidemiol, 2009. 62(4): p. 452-6.
21.          Brosschot, J.F., E. Van Dijk, and J.F. Thayer, Daily worry is related to low heart rate variability during waking and the subsequent nocturnal sleep period. Int J Psychophysiol, 2007. 63(1): p. 39-47.
22.          Beesdo, K., et al., Incidence and risk patterns of anxiety and depressive disorders and categorization of generalized anxiety disorder. Arch Gen Psychiatry, 2010. 67(1): p. 47-57.
23.          Riedel, O., et al., Frequency of dementia, depression, and other neuropsychiatric symptoms in 1,449 outpatients with Parkinson's disease. J Neurol, 2010. 257(7): p. 1073-82.
24.          Bach, J.P., et al., Health-related quality of life in patients with a history of myocardial infarction and stroke. Cerebrovasc Dis, 2011. 31(1): p. 68-76.
25.          Kessler, R.C., et al., Epidemiology of anxiety disorders. Curr Top Behav Neurosci, 2010. 2: p. 21-35.
26.          Hess, S.M. and W. Doepfner, Behavioral effects and brain amine content in rats. Arch Int Pharmacodyn Ther, 1961. 134: p. 89-99.
27.          Eccleston, D., G.W. Ashcroft, and T.B. Crawford, Effect of tryptophan administration on 5HIAA in cerebrospinal fluid in man. J Neurol Neurosurg Psychiatry, 1970. 33(2): p. 269-72.
28.          Levi, G., et al., Decrease of uptake and exchange of neurotransmitter amino acids after depletion of their synaptosomal pools. Brain Res, 1976. 103(1): p. 103-16.
29.          Kameyama, T., M. Ukai, and T. Nabeshima, Effects of various amino acids and their related agents in association with neurotransmitter substances on the morphine-induced Straub tail reaction in mice. Chem Pharm Bull (Tokyo), 1978. 26(3): p. 770-3.
30.          Hayes, R.L., L.W. Jenkins, and B.G. Lyeth, Neurotransmitter-mediated mechanisms of traumatic brain injury: acetylcholine and excitatory amino acids. J Neurotrauma, 1992. 9 Suppl 1: p. S173-87.
31.          Hauptman, M., et al., Neurotransmitter amino acids in the CNS. II. Some changes in amino acid levels in rat brain synaptosomes during and after in vitro anoxia and simulated ischemia. Brain Res, 1984. 304(1): p. 23-35.
32.          Sharp, T., S.R. Bramwell, and D.G. Grahame-Smith, Effect of acute administration of L-tryptophan on the release of 5-HT in rat hippocampus in relation to serotoninergic neuronal activity: an in vivo microdialysis study. Life Sci, 1992. 50(17): p. 1215-23.
33.          Gartside, S.E., P.J. Cowen, and T. Sharp, Effect of 5-hydroxy-L-tryptophan on the release of 5-HT in rat hypothalamus in vivo as measured by microdialysis. Neuropharmacology, 1992. 31(1): p. 9-14.
34.          Young, S.N. and S. Gauthier, Effect of tryptophan administration on tryptophan, 5-hydroxyindoleacetic acid and indoleacetic acid in human lumbar and cisternal cerebrospinal fluid. J Neurol Neurosurg Psychiatry, 1981. 44(4): p. 323-8.
35.          James, J.H., et al., Hyperammonaemia, plasma aminoacid imbalance, and blood-brain aminoacid transport: a unified theory of portal-systemic encephalopathy. Lancet, 1979. 2(8146): p. 772-5.
36.          Krause, R., et al., Plasma and brain amino acids in Walker 256 carcinosarcoma-bearing rats. Cancer Res, 1979. 39(8): p. 3065-9.
37.          Bubenik, G.A., R.O. Ball, and S.F. Pang, The effect of food deprivation on brain and gastrointestinal tissue levels of tryptophan, serotonin, 5-hydroxyindoleacetic acid, and melatonin. J Pineal Res, 1992. 12(1): p. 7-16.
38.          Wurtman, R.J., F. Hefti, and E. Melamed, Precursor control of neurotransmitter synthesis. Pharmacol Rev, 1980. 32(4): p. 315-35.
39.          Mueller, D., J.T. Porter, and G.J. Quirk, Noradrenergic signaling in infralimbic cortex increases cell excitability and strengthens memory for fear extinction. J Neurosci, 2008. 28(2): p. 369-75.
40.          Quirk, G.J. and D. Mueller, Neural mechanisms of extinction learning and retrieval. Neuropsychopharmacology, 2008. 33(1): p. 56-72.
41.          McLeod, M., et al., The heterogeneity of central benzodiazepine receptor subtypes in the human hippocampal formation, frontal cortex and cerebellum using [3H]flumazenil and zolpidem. Brain Res Mol Brain Res, 2002. 104(2): p. 203-9.
42.          Bishop, S.J., J. Duncan, and A.D. Lawrence, State anxiety modulation of the amygdala response to unattended threat-related stimuli. J Neurosci, 2004. 24(46): p. 10364-8.
43.          Walker, D.L., D.J. Toufexis, and M. Davis, Role of the bed nucleus of the stria terminalis versus the amygdala in fear, stress, and anxiety. Eur J Pharmacol, 2003. 463(1-3): p. 199-216.
44.          Pavlovsky, L., et al., Stress-induced altered cholinergic-glutamatergic interactions in the mouse hippocampus. Brain Res, 2012. 1472: p. 99-106.
45.          Griebel, G., 5-Hydroxytryptamine-interacting drugs in animal models of anxiety disorders: more than 30 years of research. Pharmacol Ther, 1995. 65(3): p. 319-95.
46.          Iversen, S.D., 5-HT and anxiety. Neuropharmacology, 1984. 23(12B): p. 1553-60.
47.          Hoyer, D., J.P. Hannon, and G.R. Martin, Molecular, pharmacological and functional diversity of 5-HT receptors. Pharmacol Biochem Behav, 2002. 71(4): p. 533-54.
48.          Krystal, J.H., et al., Glutamate and GABA systems as targets for novel antidepressant and mood-stabilizing treatments. Mol Psychiatry, 2002. 7 Suppl 1: p. S71-80.
49.          Tanaka, M., et al., Noradrenaline systems in the hypothalamus, amygdala and locus coeruleus are involved in the provocation of anxiety: basic studies. Eur J Pharmacol, 2000. 405(1-3): p. 397-406.
50.          Rudolph, U., Identification of molecular substrate for the attenuation of anxiety: a step toward the development of better anti-anxiety drugs. ScientificWorldJournal, 2001. 1: p. 192-3.
51.          Nazar, M., M. Jessa, and A. Plaznik, Benzodiazepine-GABAA receptor complex ligands in two models of anxiety. J Neural Transm, 1997. 104(6-7): p. 733-46.
52.          Beaufour, C.C., et al., Extracellular serotonin is enhanced in the striatum, but not in the dorsal hippocampus or prefrontal cortex, in rats subjected to an operant conflict procedure. Behav Neurosci, 2001. 115(1): p. 125-37.
53.          Ishida, Y., et al., Conditioned-fear stress increases Fos expression in monoaminergic and GABAergic neurons of the locus coeruleus and dorsal raphe nuclei. Synapse, 2002. 45(1): p. 46-51.
54.          Wang, Q.P., H. Ochiai, and Y. Nakai, GABAergic innervation of serotonergic neurons in the dorsal raphe nucleus of the rat studied by electron microscopy double immunostaining. Brain Res Bull, 1992. 29(6): p. 943-8.
55.          Beaufour, C.C., et al., Extracellular dopamine in the rat prefrontal cortex during reward-, punishment- and novelty-associated behaviour. Effects of diazepam. Pharmacol Biochem Behav, 2001. 69(1-2): p. 133-42.
56.          Menard, J. and D. Treit, Effects of centrally administered anxiolytic compounds in animal models of anxiety. Neurosci Biobehav Rev, 1999. 23(4): p. 591-613.
57.          Drugan, R.C., et al., Low doses of muscimol produce anticonflict actions in the lateral septum of the rat. Neuropharmacology, 1986. 25(2): p. 203-5.
58.          Owens, M.J., et al., The effects of alprazolam on corticotropin-releasing factor neurons in the rat brain: acute time course, chronic treatment and abrupt withdrawal. J Pharmacol Exp Ther, 1991. 258(1): p. 349-56.
59.          Engler, M.B. and M.M. Engler, Assessment of the cardiovascular effects of stress. J Cardiovasc Nurs, 1995. 10(1): p. 51-63.
60.          Overmier, J.B. and R. Murison, Anxiety and helplessness in the face of stress predisposes, precipitates, and sustains gastric ulceration. Behav Brain Res, 2000. 110(1-2): p. 161-74.
61.          Uchino, B.N., et al., Individual differences in cardiac sympathetic control predict endocrine and immune responses to acute psychological stress. J Pers Soc Psychol, 1995. 69(4): p. 736-43.
62.          Navarro, J.F., E. Buron, and M. Martin-Lopez, Anxiogenic-like activity of L-655,708, a selective ligand for the benzodiazepine site of GABA(A) receptors which contain the alpha-5 subunit, in the elevated plus-maze test. Prog Neuropsychopharmacol Biol Psychiatry, 2002. 26(7-8): p. 1389-92.
63.          Kirby, L.G., et al., Distinguishing characteristics of serotonin and non-serotonin-containing cells in the dorsal raphe nucleus: electrophysiological and immunohistochemical studies. Neuroscience, 2003. 116(3): p. 669-83.
64.          Miura, H., H. Qiao, and T. Ohta, Attenuating effects of the isolated rearing condition on increased brain serotonin and dopamine turnover elicited by novelty stress. Brain Res, 2002. 926(1-2): p. 10-7.
65.          Zangrossi, H., Jr., M.B. Viana, and F.G. Graeff, Anxiolytic effect of intra-amygdala injection of midazolam and 8-hydroxy-2-(di-n-propylamino)tetralin in the elevated T-maze. Eur J Pharmacol, 1999. 369(3): p. 267-70.
66.          Young, S.N., M. Leyton, and C. Benkelfat, Pet studies of serotonin synthesis in the human brain. Adv Exp Med Biol, 1999. 467: p. 11-8.
67.          Van Praag, H.M., 5-HT-related, anxiety- and/or aggression-driven depression. Int Clin Psychopharmacol, 1994. 9 Suppl 1: p. 5-6.
68.          Baer, K., et al., Postsynaptic clustering of gamma-aminobutyric acid type A receptors by the gamma3 subunit in vivo. Proc Natl Acad Sci U S A, 1999. 96(22): p. 12860-5.
69.          Crestani, F., et al., Decreased GABAA-receptor clustering results in enhanced anxiety and a bias for threat cues. Nat Neurosci, 1999. 2(9): p. 833-9.
70.          Swanson, C.J., et al., Metabotropic glutamate receptors as novel targets for anxiety and stress disorders. Nat Rev Drug Discov, 2005. 4(2): p. 131-44.
71.          Colmenares, J.L. and R.J. Wurtman, The relation between urinary 5-hydroxyindoleacetic acid levels and the ratio of tryptophan to other large neutral amino acids placed in the stomach. Metabolism, 1979. 28(8): p. 820-7.
72.          Brookes, N., Interaction between the glutamine cycle and the uptake of large neutral amino acids in astrocytes. J Neurochem, 1993. 60(5): p. 1923-8.
73.          Huang, S.C., et al., Distribution volume of radiolabeled large neutral amino acids in brain tissue. J Cereb Blood Flow Metab, 1998. 18(12): p. 1288-93.
74.          Benkelfat, C., et al., The validity of the PET/alpha-[11C]methyl-L-tryptophan method for measuring rates of serotonin synthesis in the human brain. Neuropsychopharmacology, 1999. 21(1): p. 153-7.
75.          Diksic, M., M. Leyton, and C. Benkelfat, Is alpha-methyl-L-tryptophan a good tracer for brain serotonin synthesis measurements, and does the lumped constant vary in different structures of the rat brain? J Neurochem, 1999. 73(6): p. 2621-4.
76.          D'Eufemia, P., et al., Low serum tryptophan to large neutral amino acids ratio in idiopathic infantile autism. Biomed Pharmacother, 1995. 49(6): p. 288-92.
77.          Fernstrom, J.D., Diet-induced changes in plasma amino acid pattern: effects on the brain uptake of large neutral amino acids, and on brain serotonin synthesis. J Neural Transm Suppl, 1979(15): p. 55-67.
78.          Eriksson, T. and A. Carlsson, Beta-adrenergic control of brain uptake of large neutral amino acids. Life Sci, 1988. 42(17): p. 1583-9.
79.          Agharanya, J.C. and R.J. Wurtman, Effect of acute administration of large neutral and other amino acids on urinary excretion of catecholamines1,2. Life Sci, 1982. 30(9): p. 739-46.
80.          Ablett, R.F., et al., Free tyrosine levels of rat brain and tissues with sympathetic innervation following administration of L-tyrosine in the presence and absence of large neutral amino acids. J Nutr, 1984. 114(5): p. 835-9.
81.          Brookes, N., Effect of intracellular glutamine on the uptake of large neutral amino acids in astrocytes: concentrative Na(+)-independent transport exhibits metastability. J Neurochem, 1992. 59(1): p. 227-35.
82.          Cangiano, C., et al., Brain microvessels take up large neutral amino acids in exchange for glutamine. Cooperative role of Na+-dependent and Na+-independent systems. J Biol Chem, 1983. 258(14): p. 8949-54.
83.          Adham A, H.S., Horie K, Kim M, Hatta H, Yokogoshi H., Relaxation and immunity enhancement effects of GABA administration in humans. BioFactors, 2006. 26: p. 201-208.
84.          Yokogoshi, H., GABA: Amino Acid, Peptide and Protein, in Development and Perspectives of Anti-stress Food. 2010, CMC Publishing 2006.
85.          Savic, M.M., et al., PWZ-029, a compound with moderate inverse agonist functional selectivity at GABA(A) receptors containing alpha5 subunits, improves passive, but not active, avoidance learning in rats. Brain Res, 2008. 1208: p. 150-9.
86.          Maubach, K., GABA(A) receptor subtype selective cognition enhancers. Curr Drug Targets CNS Neurol Disord, 2003. 2(4): p. 233-9.
87.          Chambers, M.S., et al., Identification of a novel, selective GABA(A) alpha5 receptor inverse agonist which enhances cognition. J Med Chem, 2003. 46(11): p. 2227-40.
88.          Milic, M., et al., PWZ-029, an inverse agonist selective for alpha(5) GABAA receptors, improves object recognition, but not water-maze memory in normal and scopolamine-treated rats. Behav Brain Res, 2013. 241: p. 206-13.
89.          Koga, Y., Does GABA's relaxing effects improve students' learning efficiency and bring better test results? Pharma Foods International Company REport, 2007.
90.          Vuong, Q.V., et al., Optimum conditions for the water extraction of L-theanine from green tea. J Sep Sci, 2011. 34(18): p. 2468-74.
91.          Yokogoshi, H., et al., Effect of theanine, r-glutamylethylamide, on brain monoamines and striatal dopamine release in conscious rats. Neurochem Res, 1998. 23(5): p. 667-73.
92.          Takeda, A., et al., Unique induction of CA1 LTP components after intake of theanine, an amino acid in tea leaves and its effect on stress response. Cell Mol Neurobiol, 2012. 32(1): p. 41-8.
93.          Tamano, H., et al., Preventive effect of theanine intake on stress-induced impairments of hippocamapal long-term potentiation and recognition memory. Brain Res Bull, 2013. 95: p. 1-6.
94.          Yamada, T., et al., Effects of theanine, a unique amino acid in tea leaves, on memory in a rat behavioral test. Biosci Biotechnol Biochem, 2008. 72(5): p. 1356-9.
95.          Sugiyama, T., et al., Inhibition of glutamate transporter by theanine enhances the therapeutic efficacy of doxorubicin. Toxicol Lett, 2001. 121(2): p. 89-96.
96.          Kimura, R. and T. Murata, Influence of alkylamides of glutamic acid and related compounds on the central nervous system. I. Central depressant effect of theanine. Chem Pharm Bull (Tokyo), 1971. 19(6): p. 1257-61.
97.          Yokogoshi, H., M. Mochizuki, and K. Saitoh, Theanine-induced reduction of brain serotonin concentration in rats. Biosci Biotechnol Biochem, 1998. 62(4): p. 816-7.
98.          Lu, K., et al., The acute effects of L-theanine in comparison with alprazolam on anticipatory anxiety in humans. Hum Psychopharmacol, 2004. 19(7): p. 457-65.
99.          Unno, K., et al., Anti-stress effect of theanine on students during pharmacy practice: positive correlation among salivary alpha-amylase activity, trait anxiety and subjective stress. Pharmacol Biochem Behav, 2013. 111: p. 128-35.
100.        Ito K, N.Y., Aoi N, Effects of L- theanine on the release of alpha-brain waves in human volunteers. Nippon Nogeikagaku Kaishi, 1998. 72: p. 153-157.
101.        Song CH, J.J., Oh JS, Kim KS., Effects of Theanine on the Release of Brain Alpha Wave in Adult Males.  . Korean Journal of Nutrition, 2003. 26(9): p. 918-923.
102.        Kimura, K., et al., L-Theanine reduces psychological and physiological stress responses. Biol Psychol, 2007. 74(1): p. 39-45.
103.        Satoh, A., et al., SIRT1 promotes the central adaptive response to diet restriction through activation of the dorsomedial and lateral nuclei of the hypothalamus. J Neurosci, 2010. 30(30): p. 10220-32.
104.        Dali-Youcef, N., et al., Sirtuins: the 'magnificent seven', function, metabolism and longevity. Ann Med, 2007. 39(5): p. 335-45.
105.        Sasaki, Y., T. Araki, and J. Milbrandt, Stimulation of nicotinamide adenine dinucleotide biosynthetic pathways delays axonal degeneration after axotomy. J Neurosci, 2006. 26(33): p. 8484-91.
106.        Gong, B., et al., Nicotinamide riboside restores cognition through an upregulation of proliferator-activated receptor-gamma coactivator 1alpha regulated beta-secretase 1 degradation and mitochondrial gene expression in Alzheimer's mouse models. Neurobiol Aging, 2013. 34(6): p. 1581-8.
107.        Donmez, G., et al., SIRT1 suppresses beta-amyloid production by activating the alpha-secretase gene ADAM10. Cell, 2010. 142(2): p. 320-32.
108.        Gericke, N. and A.M. Viljoen, Sceletium--a review update. J Ethnopharmacol, 2008. 119(3): p. 653-63.
109.        Hirabayashi M, I.k., Clinical application of South African tea on dementia dog. 23e, 2002. 21(2).
110.        Hirabayashi M, I.k., Clinical application of South African tea for cat. Japanese Journal of Small Animal Practic, 2004. 23(2).
111.        Nell, H., et al., A randomized, double-blind, parallel-group, placebo-controlled trial of Extract Sceletium tortuosum (Zembrin) in healthy adults. J Altern Complement Med, 2013. 19(11): p. 898-904.
112.        Harvey, A.L., et al., Pharmacological actions of the South African medicinal and functional food plant Sceletium tortuosum and its principal alkaloids. J Ethnopharmacol, 2011. 137(3): p. 1124-9.
113.        Cashman, J.R., et al., Stereoselective inhibition of serotonin re-uptake and phosphodiesterase by dual inhibitors as potential agents for depression. Bioorg Med Chem, 2009. 17(1): p. 337-43.
114.        Terburg, D., et al., Acute effects of Sceletium tortuosum (Zembrin), a dual 5-HT reuptake and PDE4 inhibitor, in the human amygdala and its connection to the hypothalamus. Neuropsychopharmacology, 2013. 38(13): p. 2708-16.
115.        Korff, S., D.J. Stein, and B.H. Harvey, Cortico-striatal cyclic AMP-phosphodiesterase-4 signalling and stereotypy in the deer mouse: attenuation after chronic fluoxetine treatment. Pharmacol Biochem Behav, 2009. 92(3): p. 514-20.
116.        Miclo, L., et al., Characterization of alpha-casozepine, a tryptic peptide from bovine alpha(s1)-casein with benzodiazepine-like activity. FASEB J, 2001. 15(10): p. 1780-2.
117.        Messaoudi, M., et al., Effects of a tryptic hydrolysate from bovine milk alphaS1-casein on hemodynamic responses in healthy human volunteers facing successive mental and physical stress situations. Eur J Nutr, 2005. 44(2): p. 128-32.
118.        Kim, J.H., et al., Efficacy of alphas1-casein hydrolysate on stress-related symptoms in women. Eur J Clin Nutr, 2007. 61(4): p. 536-41.

No comments:

Post a Comment